CLINICAL-MORPHOLOGICAL AND GENETIC CHARACTERISTICS OF ENDOMETRIAL HYPERPLASTIC PROCESSES

N.L. Tsyndrenko; M.S. Lyndin; K.O. Sikora; A.V. Shamray; D.G. Tsepochko; Yu.M. Lyndina; A.M. Romaniuk

doi:10.34921/amj.2024.3.013

Authors

N.L. Tsyndrenko Sumy State University
M.S. Lyndin Sumy State University
K.O. Sikora Sumy State University
A.V. Shamray Sumy State University
D.G. Tsepochko Sumy State University
Yu.M. Lyndina Sumy State University
A.M. Romaniuk Sumy State University

DOI:

https://doi.org/10.34921/amj.2024.3.013

Keywords:

cyclooxygenase-2, endometrial hyperplasia, endometrial polyps, estrogen receptor, hyperplastic processes of the endometrium, PvuII polymorphism

Abstract

The article presents data on the clinical, morphological, and genetic characteristics of endometrial hyperplastic processes (EHP), which involve different histological variants. Tissue samples of ninety-five patients obtained post-surgery via hysteroresectoscopy underwent immunohistochemical analysis for estrogen receptor alpha (ERα) and cyclooxygenase-2 (COX-2) expression. Polymerase chain reaction (PCR) followed by restriction fragment length analysis (RFLP) was employed to investigate the PvuII polymorphism of the ESR1 gene (rs2234693). Significantly elevated estrogen (ER) expression was noted in the epithelial component compared to the stromal component (p < 0.05). However, no significant differences were observed in ER expression among different histological variants of EHP (p > 0.05). COX-2 expression was uniformly detected in the epithelial component of all EHP tissue samples. Notably, no association was found between the PvuII polymorphism of the ESR1 gene and EHP development, nor with specific histological subtypes or expression levels of ERα and COX-2.