THE INFLUENCE OF HUMAN PLACENTA HYDROLYSATE ON REGENERATION PROCESSES IN INTESTINAL ANASTOMOSES

Abstract

The article presents information about experimentally study of the effect of human placenta hydrolysate on regeneration processes in primary intestinal anastomoses against the background of simulated acute intestinal obstruction. For this purpose, experimental studies were carried out on 60 rabbits, of which 20 rabbits constituted the control group, and 40 rabbits - the main group. The main group, in turn, was divided into 2 subgroups. First, a model of strangulated acute intestinal obstruction was created, and a day later relaparatomy was performed, during which the gangrenous segment of the intestine was resected within healthy tissues and a primary single-row entero-enteroanastomosis was performed side to side. While in the control group, standard treatment was used, and in the main group, in addition to standard treatment, human placenta hydrolysate was administered at a dose of 0.15 ml / kg (8.4 mg / kg) once a day. In the first subgroup, the drug was injected intramuscularly, and in the second subgroup - retroperitoneally through a catheter placed in the root of the mesentery of the small intestine. Intestinal anastomoses were resected and sent for morphological examination 3.5, 7 and 15 days after surgery. It was found that the index of epithelial mitosis, fibroblastic activity, the formation of collagen fibers de novo and the density of microvessels increased by an average of 10-20% more in animals of I and II subgroups of the main group, compared with the control group during the indicated periods, this in turn indicates that the regenerative processes in the area of ​​the anastomosis in animals of the main group proceed more intensively. The manifestations of regenerative parameters were noted earlier and were more intense with the introduction of placenta hydrolysate into the mesentery root of the small intestine than with intramuscular administration. In this regard, according to the authors the use of human placenta hydrolysate is advisable in patients with a high risk of intestinal anastomotic leakage.