METHOD OF STUDYING THE GREATER OMENTUM OF WHITE RATS IN SEPTIC PERITONITIS

Abstract

The article presents the methodology for creating a septic peritonitis model in white rats and investigates the reactive changes in the greater omentum at the site of inflammation. The study was conducted on 15 sexully mature male white rats with a body weight 276±6.56 g. Septic peritonitis was modeled as follows: under sterile conditions and ether anesthesia, experimental animals underwent a midline laparotomy (2-3 cm in length), the apex of the cecum was localized and exteriorized through the incision; using a G16 injection needle, a perforation was made in the anterior wall of the cecum, and the intestinal contents were removed with a gauze pad, after which the cecum was repositioned into the abdominal cavity and the abdominal wall was then sutured using 2/0 capron sutures. The study demonstrated that proposed method for studying the greater omentum of white rats in septic peritonitis involves the initiation of intraperitoneal septic inflammation of the peritoneu. The suggested experimental approach is technically simple, standardized, quick, and cost-effective, making it suitable for use in relevant research studies.