INTERDEPENDENCE BETWEEN MICROELEMENTS AND IMMUNE SYSTEM INDICATORS AND THYROID STATUS IN CHILDREN WITH AUTOIMMUNE THYROIDITIS

Abstract

The article provides information of the study of the correlation between the levels of microelements (selenium, zinc, copper) in the blood serum, iodine in the urine, immune parameters (proinflammatory cytokines: TNF-α, IL-1β, IL-6, T cells: CD3+, CD4+, CD8+, CD19+), thyroid hormones (TSH, fT3, fT4), thyroid antibodies (Anti-TPO, Anti-TQ). The study included 83 children diagnosed with autoimmune thyroiditis. The level of selenium in the blood serum of children was measured on a Varian AA240-Duo Zeeman atomic absorption spectrometer, copper and zinc were determined by the colorimetric method on a StatFax device, iodine in urine – by a sensitive and selective photometric method, immune indicators – T (CD3+), Telcher/ inducer (CD4+) and B-lymphocytes (CD19+), T-suppressor-cytotoxic (CD8+) lymphocytes – by flow cytometry, inflammatory cytokines interleukin-1 (IL-1), interleukin-6 (IL-6) and TNF-α – by solid-phase ELISA, free thyroxine (fT4), triiodothyronine (fT3) and thyroid-stimulating hormone (TSH) – by radioimmunoassay (RIA). Hemagglutination reaction was used to determine antibodies (Anti-TPO and Anti-TQ). Research has revealed a positive correlation between levels of microelements and immune parameters, cytokines, thyroid hormones and thyroid antibodies. An inverse correlation was observed between selenium concentration in the blood serum, proinflammatory cytokines and thyroid antibodies.