VAC-THERAPY IN THE TREATMENT OF LOWER LIMB CHRONIC WOUNDS. ANALYSIS OF CLINICAL AND BIOFILM DATA

I.V. Korpusenko; N.N. Nor; V.P. Kryshen; N.V. Trofimov; E.G. Garkava; E.I. Korpusenko; A.N. Makarenko; V.E. Kudryavtseva; V.V. Koshtura

doi:10.34921/amj.2023.4.008

Authors

I.V. Korpusenko Dnipro State Medical University
N.N. Nor Dnipro State Medical University
V.P. Kryshen Dnipro State Medical University
N.V. Trofimov Dnipro State Medical University
E.G. Garkava National Aviation University
E.I. Korpusenko Dnipro State Medical University
A.N. Makarenko Interregional Academy of Personnel Management
V.E. Kudryavtseva Pridneprovsk State Academy of Physical Culture and sports
V.V. Koshtura Dnipro State Medical University

DOI:

https://doi.org/10.34921/amj.2023.4.008

Keywords:

biofilms, chronic wound, VAC therapy, surgical treatment

Abstract

The article presents the results of a study conducted to investigate the effects of negative atmospheric pressure on the biofilms of chronic wounds. A total of 62 patients with chronic wounds of the lower extremities, aged 45 to 75 years, were examined and treated. Microorganism identification was performed using MALDI-TOF spectrometry (Bruker, Germany). The quantitative expression of biofilm formation was represented by optical density values measured on the StatFax2100 photometer.Microbial biofilms were detected in 72% of patients with chronic wounds. The causative agents of chronic infection included representatives of both Gram-positive and Gram-negative flora, with a tendency for Staphylococcus species to predominate. VAC therapy, in combination with surgical treatment and antibiotic therapy, prevented the reformation of biofilm, hindered further wound infection, leading to a radical resolution of the wound process, and resulted in wound healing in 100% of treated patients.