ERYPTOSIS IN PATIENTS WITH GESTATIONAL DIABETES MELLITUS AND HYPERTENSIVE DISORDERS OF PREGNANCY

Abstract

The study was conducted to analyze eryptosis indices and the presence of cell membrane alteration of circulating erythrocytes in patients with gestational hypertension and diabetes mellitus. This study included 57 pregnant patients, which were divided into four distinct groups: 15 women with gestational diabetes mellitus (GDM), 15 women with pregestation diabetes mellitus (DM), 15 women with hypertensive disorders of pregnancy (HDP) and 12 pregnant women without extragenital and obstetric pathology. Staining of erythrocytes with annexin V-FITC and 2′,7′-dichlorodihydrofluorescein diacetate was used to assess the degree of eryptosis collected from the patients. Fluorescence was detected on a BD FACSCanto™ II Cell Analyzer. Cell membranes of erythrocytes were assessed using a fluorescent probe O1O (2-(2¢- hydroxy-phenyl)-5-phenyl-1,3-oxazole). Fluorescence in red blood cell suspensions was acquired on a “PerkinElmer FL8500” fluorescence spectrometer. GDH and DM were associated with eryptosis activation, evidenced by an increased phosphatidylserine externalization and excessive reactive oxygen species (ROS) generation, against the background of cell membrane alterations, namely a decrease in the lipid order. GDM was accompanied by neither membrane changes, no eryptosis activation. Thus, the results of the study show that ROS-dependent eryptosis and red blood cell membrane alterations are observed in gestational hypertension.