STUDY OF THE ANTIPARASITIC EFFECT OF CONIFEROUS CHLOROPHYLL-CAROTENE PASTE TO LEISHMANIA

Abstract

The article provides information on the research work conducted to study the antiparasitic effect of chlorophyll-carotene paste produced from coniferous plants against Leishmania promastigotes and to investigate the effectiveness of this drug in the treatment of leishmaniasis. In experiments, the microculture method was used to obtain the pure culture of the promastigotes. For this purpose, a pure culture of Leishmania promastigotes was obtained by microculture from materials taken from patients with cutaneous leishmaniasis (from skin wounds).

In the II phase of the study, No. 1 (glucantim), No. 2 (coniferous chlorophyll-carotene paste) and control (vaseline oil) were made in different dilutions (1x10, 1x20, 1x40 and 1x80) and used against to promastigotes of Leishmania. The antiparasitic (lethal) effect of the substances was evaluated on the basis of the loss of mobility of promastigotes.

At the result of the experiments, it was clear that coniferous chlorophyll-carotene paste had an anti-parasitic effect on promastigotes at a 1:2 dilution after 60 minutes, at a 1:4 dilution for 2 hours, at a 1:8 dilution for 3 hours. The lethal effect was recorded based on the loss of mobility of promastigotes. The study has confirmed the antiparasitic properties of coniferous chlorophyll-carotene paste.