THE STATE OF THE PARIETAL MICROFLORA OF THE LARGE INTESTINE IN PATIENTS WITH IRRITABLE BOWEL SYNDROME AND WITH DIARRHEA PREVALENCE

Abstract

The article presents the results of a study conducted to obtain information about the composition of the mucosal microflora of the large intestine in the presence of a clinical form of irritable bowel syndrome with a predominance of diarrhea. The study included 30 patients, 19 men and 11 women, aged 18 to 64 years, with a mean age of 42.3 ± 4.7 years. All subjects met the Rome criteria for irritable bowel syndrome, version IV. All patients underwent total colonoscopy with stepwise biopsy of the mucosa at the level of the sigmoid, transverse colon and cecum. The obtained biopsies were homogenized and mixed with 0.1 ml of 0.9% sodium chloride solution, and then serial dilutions 10¹-10⁹ were prepared and inoculated on the surface of the different nutrients. Disturbances in the composition of the parietal microflora were detected in 90% of the examined. In this case, the most often diagnosed II and I degrees of dysbiosis (40% and 33.3% of cases, respectively), followed by the III degree in 26.6% of cases. The average concentrations of microorganisms that form the indigenous fraction of the parietal microbiota of the large intestine had significantly lower values compared to those in the control group of individuals without signs of gastrointestinal tract pathology: bifidobacteria - 5.12x10⁶ CFU/g, lactobacilli - 3.87x10⁶ CFU/g, Escherichia coli - 5.59x10⁷ CFU/g. The growth of opportunistic microflora representatives in the group of patients with irritable bowel syndrome as a whole was recorded in 76,6 % of cases. The most frequently observed increase in the concentration of opportunistic microorganisms such as Candida, Proteus and Staphylococcus aureus.