INFLUENCE OF ANTITUMOR CHEMOTHERAPEUTICS ON MECHANICAL PROPERTIES OF INJURED TUBULAR SKELETAL BONE

T.V. Riabenko; S.M. Dmytruk; O.S. Yarmolenko; M.S. Pernakov; Т.P. Teslyk; A.A. Ponyrko; О.V. Gordienko

doi:10.34921/amj.2022.1.019

Authors

T.V. Riabenko Medical Institute, Sumy State University https://orcid.org/0000-0003-2740-389X
S.M. Dmytruk Medical Institute, Sumy State University
O.S. Yarmolenko Medical Institute, Sumy State University
M.S. Pernakov Medical Institute, Sumy State University
Т.P. Teslyk Medical Institute, Sumy State University
A.A. Ponyrko Medical Institute, Sumy State University
О.V. Gordienko Medical Institute, Sumy State University

DOI:

https://doi.org/10.34921/amj.2022.1.019

Keywords:

bone fractures, microhardness, strength, antitumor chemotherapeutics

Abstract

The aim of the study was to investigate the effect of antitumor chemotherapeutics on the mechanical properties of the injured long skeletal bone.

The research was carried out on 96 laboratory rats, which were inflicted with a perforated defect with a diameter of 2 mm to the bone marrow canal in the middle third of the femoral shaft. Animals were divided into the control group (n = 24), as well as three experimental (I, II, III, n = 72) ones, which were injected after injurywith theantitumor chemotherapeutics intraperitoneally: I group – with doxorubicin (60 mg/m²),
II – with 5-fluorouracil (600 mg/m²), III – with methotrexate (40 mg/m²). Re-administration of appropriate chemotherapeutics was performed in the same doses on each 21st day throughout the experiment. On the 15th, 30th, 45th, and 60th day after injury, the compressive strength limit of the bones and the microhardness of the regenerate were determined.

Anticancer chemotherapy drugs reduce the compressive strength of the bone. On the 60th day after injury, doxorubicin reduces this indicator by 40.08%, 5-fluorouracil – by 40.93%, methotrexate – by 35.19% compared with the control. Antitumor drugs slow down the growth of bones in width: doxorubicin – by 18.54%, 5-fluorouracil – by 10.24%, methotrexate – by 17.40% compared with the control on the 60th day after injury. Antitumor chemotherapy also reduces the microhardness of the femur in the regenerate area: under the influence of doxorubicin by 37.32% (p˂0.05), 5-fluorouracil – by 41.47% (p˂0.05),
methotrexate – by 35.83 % (р˂0.05) in comparison with the control group.

The use of antitumor chemotherapeutics causes a decrease in bone strength and microhardness in the area of bone regeneration, as well as slows bone growth. The most pronounced negative effect on the tensile strength of injured tubular bones has 5-fluorouracil; doxorubicin has the greatest inhibitory effect on bone growth in thickness.