THE MUTUAL RELATIONSHIP BETWEEN INTESTINAL MICROBIOTAS LEVEL AND CYTOKINE STATUS IN CHILDREN WITH ACUTE URTICARIA

Abstract

The article provided information about the research performed for purposes of the study of the mutual relationship between intestinal microbiotas level and cytokine status in children with acute urticaria. 80 children with acute urticaria were included in the study. 20 practical healthy children were included in the control group. IL-9, IL-17, and TNF-β concentrations were determined in all children by the immunosorbent assay method (ELISA). Extraction of intestinal microbiotas (bifidobacterium spp, E.Coli, B.fragilis, C.difficile, Lactobacillus spp, total number of bacterias) was performed by manual isolation reagent of Qiagen firm. Amplification was performed by PCR method in CFX 96 device. The study results showed that the IL-9 and IL-17 concentrations were increased 4 times and 1.5 times, respectively compared to the control group, and no significant difference was determined in TNF-β concentration in comparison with the control group results. In the comparison of the frequency of occurrence of various toxins of microorganisms in the large intestine, the differences were determined in Bifidobacterium spp, E.Coli, and B. fragilis microorganisms. In cases of changes revealed in the intestinal microbiota, it provides doctors prescribe symbiotics for the correction of intestinal microflora. Various inter-systematic mutual relations were determined in the case of acute urticaria disease in children. The results showed correlation among clinical signs of acute urticaria disease, types of intestinal microbiota and high levels of IL-9, IL-17.